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Given the low-internalization rate of the β1AR, this effect could not be quantified by cell fractionation.
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HSV infection was quantified by cell surface expression of HSV gD two days post-infection.
Reprogramming was quantified by cell survival using Alamar Blue or counting alkaline phosphatase-positive colonies.
RPE cells that invaded the lower surface of the membrane were quantified by cell counting (320 × magnification).
The nucleosomal DNA degradation was quantified by Cell Death Detection ELISA kit using antihistone antibody (Roche, Mannheim, Germany) as described previously [ 18].
The fluorescence can be quantified by fluorescence activated cell sorting (FACS) analysis or by special microscopic software.
Cell interfaces of a Henle's loop cell were quantified by counting cells directly contacting that cell.
The percentage of apoptotic cells was quantified by analyzing cell morphology of the GFP-positive cells.
The adhesion Y79 cells were quantified by the Cell Counting Kit-8 assay.
The adhesion Y79 cells were quantified by the Cell Counting Kit-8 assay according to the manufacturer's instructions.
The heart and lungs were isolated, and cell dissemination was quantified by counting cell clusters using confocal microscopy at 10X (NA = 0.45) with a step distance of 1 µm.
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