Expression of β-galactosidase was quantified by adding 400 µl Z-buffer contaning 1 mg/ml ONPG to 100 µl of each sample as described previously [36].
Samples were centrifuged and the haemoglobin content was quantified by adding equal amount of TMB to the supernatant.
Again, the amount of bound antibody was quantified by adding an AP-labelled secondary anti-human IgG antibody.
Spontaneous release was quantified by adding T-cell medium alone to targets and maximal release by adding 2% Triton to targets.
Cel-miR-39 was quantified by adding 2 μl of 10-fold diluted cDNA (not pre-amplified) in real-time PCRs as described above.
According to Wood and Bhat [ 101], low reducing sugar concentrations were quantified by adding 1.25 g/L glucose to the samples.
The concentration dependence of the effects of flupirtine on tonically active GABAA receptors was quantified by adding bicuculline methiodide to block tonic GABAergic currents.
The "net benefit" value of the forecast can be quantified by determining the added financial value of these better informed decisions (Mason, [1966]).
Gendreau cautions this adds an element of doubt that can not be quantified by mathematical models.
But perhaps the biggest problems facing Hershey cannot be quantified by dollar figures.
