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Based on CyTOF® data, immune cell populations can be quantified at single-cell resolution according to their phenotype and can be defined using over 30 parameters.
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More recently, it has been demonstrated that by acquiring diffraction phase microscope (DPM) measurements at different wavelengths, hemoglobin concentration may be quantified at the single cell level [ 25].
To test whether MKRN1 is disproportionally expressed between the OCT4+ and OCT4− subpopulation, the mean fluorescent intensity of MKRN1 in the OCT4+ and OCT4− fraction of ESC populations cultured for 72 h in self-renewal (+LIF) or differentiation conditions (−LIF or +RA) was quantified at the single-cell level using flow cytometry.
Fluorescence intensity in daughter and mother cells was quantified at the first cell division.
We describe here the genotoxicity induced by MA as observed by comet assays, in which chromatin integrity is quantified at the level of single cells.
To further investigate the effect of collagen I and laminin on proliferation of HSC-T6 cells, cell numbers were quantified at different time points over the period of a single passage.
The viability of cells was quantified at 24, 48, 72, and 96 h following LTP treatment.
Cell survival was quantified at different time points using 3- 4,5-dimethylthiazole-2-yl -2,5-diphenyl tetrazolium bromide (MTT) at 595 nm.
The total cell number was quantified at 24 h intervals up to 6 days.
A PKB substrate was introduced into single cells, and the formation of phosphorylated product was quantified at varying times and substrate concentrations.
Infiltrated endothelial cells and macrophages in Matrigel matrices were quantified in a single-cell suspension by fluorescence-activated cell sorting (FACS) (Figure 1B, Figure S1A).
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