When this system is programed with a truncated mRNA that lacks a stop codon, the ribosome translates to the end of the transcript and stalls, creating a synchronized population of ribosome nascent chain complexes (RNCs) of defined length that functionally engage the targeting and translocation machinery (Gilmore et al., 1991).
The Ground Control was initiated with a precise 6 day delay to enable the OES environment to be programed with those environmental conditions that can be replicated on the ground, such as cabin temperature and CO2 levels, taken from ISS telemetry.
Translation reactions (100 μl) were programed with mRNA templates encoding an N-terminal 3x-FLAG tag or 3x-HA tag (control) followed by the first 126 residues of TNFα.
Data is entered at the time of interview on a dedicated laptop computer into a Microsoft Access database which is programed with skip patterns and range check functions.
The OES was programed with the same environmental conditions experienced on orbit, and the Ground Control was executed with a 6 day delay.
The VR games were programed with the OpenSceneGraph API [ 59], which allows animating deformable virtual objects and creating scenes with dynamic simulations using the Bullet Physics package.
With appropriate firmware, cheap sensor devices can also be programed into a swept spectrum analyzer.
The GC oven temperature was programed from 50 °C with a 2 min hold, increased at 5 °C min−1 to 260 °C, 3 min hold, and then at 20 °C min−1 to 300 °C, final hold 5.9 min; total run time 54.9 min.
A simulation was programed that created a data set with 4,350 genes, of which 70 were selected randomly as zinc responsive.
Next, a multi-dimensional normalization map generation algorithm is programed to generate a multi-dimensional normalisation map.
