Sentence examples for be produced in soluble from inspiring English sources

Although the majority of hER-E/F protein was produced in insoluble form under standard bacterial culture conditions, hER-E/F could be produced in soluble, biologically active form by altering the sequence of the expressed protein and by varying the host culture conditions.

We fused xarB to the 5' end of xynA2 in pHsh-xynA2 to examine whether a multifunctional xylan degrading enzyme could be produced in soluble form.

In the case of latherin, we are fortunate that it can be produced in soluble recombinant form in bacteria, and we have already obtained high quality protein NMR spectra as a preliminary to solving its solution structure (R.E. MacDonald, A. Cooper, B.O. Smith and M.W. Kennedy, unpublished).

This efficiency is remarkable as most of the constructs used in this study encode proteins that are difficult to be produced in soluble form.

Another important consideration was the availability of the Paramecium bursaria chlorella virus SET domain protein (vSET) that catalyzes H3K27-trimethylation and can be produced in soluble and active form in E. coli [ 12, 13].

Mutated protein libraries were produced in soluble, active form by means of cell-free protein synthesis.

Recombinant HisF-C**C is produced in soluble form, forms a pure monomer with its tryptophan residues shielded from solvent and unfolds with similar cooperativity as HisF.

Evaluation of HaloTag7 for expression of 23 human proteins in Escherichia coli relative to MBP, GST and His6Tag revealed that 74% of the proteins were produced in soluble form when fused to HaloTag7 compared to 52%, 39%and22%2%, respectively, for the other tags.

The his-tagged IFBD2 construct his-IFBD22) (previously generated) was produced in soluble form using the bacterial protein expression protocol as described (Supplemental Information S1).

Very recently, the newly discovered Conotoxin lt7a was produced in soluble form in fusion with thioredoxin (Pi et al. 2007).

Proteins were produced in soluble form in the E. coli strain BL21-AI using N-terminal OmpA bacterial secretion signal from Bordetella avium [ 26].