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Here, we have shown that high quality mtDNA sequences can be produced from single molecule sequencing data.
This template/assign strategy is flexible, as templates can be produced from single samples to establish a master template; combinations of samples to produce a consensus template; or stochastic subsets of cells to optimize run times and the sensitivity of detection of small clusters.
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In contrast, the third class of small RNAs, Piwi-interacting RNAs (piRNAs), are suggested to be produced from single-stranded precursor RNAs independently of Dicer and are loaded into the Piwi subfamily of Argonaute proteins.
Electroluminescent devices have been produced from single nanotubes.
Sugarcane transplants were produced from single budded setts, cut from mature stalks in field-grown plots of commercial sugarcane of the same age and from the same field.
EST sequences are produced from single-pass sequencing of 5'- and/or 3'-end of the clones.
Genetic material required to produce each vaccine was produced from single-cell suspensions of lymphocytes taken from each patient's excised tissue and produced the same day as tissue procurement.
Importantly, we show that two miRNAs can be produced from a single precursor, either from tandem stem-loops or tandemly arrayed in a single stem-loop.
It turns out, for example, that several different proteins may be produced from a single stretch of DNA.
Unlike most other types of adult stem cells, mesenchymal cells grow well in culture, so thousands of doses can be produced from a single donation.
Hence, many replications may be produced from a single prefabricated mold using this method.
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