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It has been shown that offspring could be produced from mouse oocytes stored at room temperature in TYH, CZB-H and KSOM media with 0 to 0.5% Bovine serum albumin (BSA), not from oocytes stored at 5 or 37°C [8].
In 2003, a group headed by Hans R. Schöler reported that oocyte-like cells could be produced from mouse embryonic stem (ES) cells in vitro.
From the first work by Hübner et al. in 2003, showing that oocyte-like cells (OLCs) could be produced from mouse embryonic stem (ES) cells in vitro, derivation of germ cells from stem cells raised great public sensation, inspiring hopes in some, fears in others.
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First strand cDNA was produced from mouse total RNA according to a modified version of the Clontech SMART protocol (E. Mardis, personal communication), using approximately 1 µg of total RNA and SuperScript II (Invitrogen).
Chimeric mice were produced from mouse ES cell lines.
The scFv9C5 and scFv4B12 constructs were produced from mouse mAbs (both IgG1 κ isotype) against Z α1-antitrypsin.
Wnt3a conditioned medium was produced from mouse L-cells (ATCC) stably expressing the recombinant protein and used to treat HeLa cells.
MAbs 35A7, B17, CE25, and 192 were produced from mouse hybridoma ascites fluid by ammonium sulfate precipitation and ion-exchange chromatography.
CNBs with a diameter of 0.6 mm and a width/thickness of 1 mm were produced from mouse spleens, corresponding to a volume of tissue of about 0.28 mm (which is approximately the same volume of tissue present in a spleen section with 5 mm × 5 mm × 10 μm size, used as control).
mAb secreting hybridomas were produced from mice hyperimmune to the model Ag tobacco mosaic virus protein.
In order to generate a BC4-like monoclonal antibody, hybridomas were produced from mice immunised with the recombinant pTn28 phage lysate encoding the EGF-like repeats of human tenascin previously shown to contain the BC4 antigenic epitope.
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