In this paper we have described how parental data is processed into individual ICD-10 codes, infectious disease categories and infectious episodes in TEDDY study.
The polycistronic mRNAs are processed into individual transcripts by trans-splicing of a common capped mini-exon sequence (the spliced leader, SL) to the downstream coding sequence (CDS) and concomitant polyadenylation of the upstream gene [ 24].
In the nucleus, functionally unrelated genes are transcribed polycistronically [ 1] and are processed into individual mature transcripts by trans-splicing, acquiring a 39-nt mini-exon from the spliced leader (SL) RNA that is attached to the 5' end of each individual messaged before it can be translated.
Given that Leishmania genes are transcribed into polycistronic RNA precursors that need to be further processed into individual mRNAs by trans-splicing and polyadenylation, post-transcriptional regulation represents the main level of controlling gene expression in these parasites [ 8, 21].
PR, RT, RH, and IN are encoded in a polyprotein (Pol) that is later processed into individual polypeptides by PR.
Another potential advantage of a CRISPR/Cas system is that a single vector can accommodate multiple guide RNAs in series, which are then processed into individual RNAs that allow for simultaneous, multiplexed targeting of multiple sites in the same cell (Cong et al., 2013).
This suggests that in H. virescens and in G. mellonella, like in S. frugiperda, X-tox proteins are not processed into individual bioactive defensins.
Log files are first processed into individual excel spreadsheets for each channel (animal) in an easy-to-read format, and then loaded into a custom Excel workbook which automates data analysis (e.g, average time in each quadrant by the animal, average speed, proportion of time punished, time spent at the edge vs. center of the dish, and area of the dish explored).
Individual images were then imported into Adobe Photoshop (Adobe Systems Incorporated, San Jose, CA) so that they could be processed, eventually assembled into digital montages, and reduced to the final enlargement.
