Its recent budget proposal eliminates financing for inspections of horse slaughterhouses, which would effectively reinstate a ban on the practice in which horses are processed for human consumption.
According to the American Society for the Prevention of Cruelty to Animals (ASPCA), there are currently no U.S. slaughterhouses where horses are processed for human consumption, though these animals are shipped from some states to other countries to be killed for food.
For samples failing to satisfy these criteria, a Test3 (GeneChip®3 Array, Affymetrix) was used and only high quality cRNA samples from Test3 microarray assays were processed for human pan-genomic microarray hybridization, i.e. when backgrounds were less than 100 and the housekeeping gene 3'/5' ratio was less than 3 for GAPDH and less than 6 for β-actin.
At 3 months post-transplantation, the mice were killed and the brain tissues were processed for human-specific cell identification.
Biopsies were processed for histology and for human papillomavirus (HPV) typing.
One in six sections were processed for Nissl staining or immunostanined for human nuclei (hNUC, mouse monoclonal, 1∶200, Chemicon), human GDNF (goat polyclonal, 1∶250, R&D), and choline acetyl transferase (ChAT, goat polyclonal, 1∶200, Chemicon).
Adjacent serial coronal sections were processed for double immunofluorescence staining of human nuclear matrix antigen (hNuMA, 1∶100, mouse monoclonal, Oncogene) and antibodies specific for cell type specific markers.
Intact, euploid human embryos were processed for in situ hybridization as described elsewhere (67).
Human islets were processed for RNA within 24 h after arrival of the shipment.
