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Notably, infection of cell cultures could be prevented by expression of KDEL peptide aptamers.
This can be prevented by expression of either telomerase or by increasing the levels of LAP2α.
Alternatively, growth factor binding to the receptor can be prevented by expression of a mutant growth factor, or by sequestering the growth factor extracellularly.
Consistently, almost all zebrafish LOF phenotypes could be prevented by expression of the homologous human gene, supporting gene orthology and shared gene function at the molecular or cellular level.
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Moreover, mobility shift of PKR in SDS-PAGE was prevented by expression of E3 protein, showing that this viral product targeted PKR to block its activation.
We previously showed that cetuximab downregulates HIF-1α in cancer cells sensitive to anti-EGFR therapy through inhibition of HIF-1α protein synthesis, which was prevented by expression of a myristoylated Akt that is constitutively active [16].
Motility and invasion of numerous cancer cell types is prevented by expression of the tumour-suppressor E-cadherin.
Repeat tandem arrays containing 3G QFP sequences are inherently unstable in yeast, but this sequence instability is prevented by expression of PIF1 [ 22].
Interesting work by Vuori and colleagues showed that pY221 Crk was required for recruiting activated Rac1 to the membrane fraction, and Rac1 re-localization was prevented by expression of the Y221F Crk mutant [ 172].
Moreover, Rheb contributed to 5-HT6 receptor-dependent mTOR signalling in HEK-293 cells: both mTOR (S) and S6 (S240/244) phosphorylations elicited by WAY181187 were prevented by expression of a Rheb-dominant-negative mutant (I39K, Fig 3B).
The transition back to a more classical migratory chemotaxis to wounds correlates with the beginning of metamorphosis and is prevented by expression of dominant negative ecdysone receptor in hemocytes [ 60 ].
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