Examples of genomic segments that were present uniquely in FHH and SS, but not in any other strains, including their respective control strains, are shown in Figure 3A.
The approach is generalizable to identify any genomic sequences that are present uniquely in one sex (e.g., Y or W chromosomes within other animal species), and is potentially useful for characterizing the genomes of non-model organisms.
We speculate that RIG-I emerged earlier than MDA5/LGP2 since vertebrate RIG-Is grouped with the invertebrate progenitors rather than the MDA5 and LGP2 proteins which are present uniquely in vertebrates, in stark contrast with the evolutionary model proposed by Sarkar et al [ 16], where LGP2 preceded both MDA5 and RIG-I in evolution.
Finally, because all the elements of L I are presented uniquely in T, α and γ are two permutations on { 1, …, m }.
We have extended the traditional homozygosity mapping method by analysing the haplotype within shared ROH regions to identify homozygous segments of identical haplotype that are present uniquely or at a higher frequency in ASD probands compared to parental controls (Fig. 1).
Unlike other actin-based protrusions such as lamellipodia and filopodia that are present in normal cells, invadopodia are uniquely present in invasive cancer cells and considered as the transformed version of podosomes which are present in highly invasive normal cells such as macrophages, osteoclasts and dendritic cells.
While all the reads present in the uniquely aligned group are present in the all read group, this Pearson calculation helps to guard against potential PCR bias (i.e., over-amplification of certain library fragments over others).
Each uniquely acquired CRISPR spacer is present in the population at a different frequency due to the timing at which the resistance was originally gained [40].
While 442 individual proteins were detected in both the specific and the nonspecific eluates, and 277 proteins were uniquely present in the nonspecific eluate, 96 proteins were present in the specific eluate but absent from the nonspecific eluate.
Of these, 32%% (4,685) were uniquely present in the somatic control (blood) samples, 31 % (4,516) were uniquely present in the cancer samples and 37%% (5518) were present in both the control (blood) and the cancer samples (Fig. 1a; Additional file 2: Figure S3).
