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Induction by forskolin in vitro suggests that the factors mediating repression can be over-ridden by a strong stimulus or may not be present in vitro (i.e. afferents).
However, proteins identified in vivo (Lendenmann et al. 2000; Yao et al. 2001) were also shown to be present in vitro, thus validating the system used in the current study.
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While the SCN innervates the MBH region [53], endogenous rhythms in clock gene expression are present in vitro in the MBH and pituitary gland [44], [46], [47], [65], tissues critical for the regulation of metabolism [49].
GS expression, which is present in vitro (Fig. 1), was absent in the transplanted cells in the periportal areas (Fig. 3G).
Both exosomes and shedding vesicles are present in vitro and in vivo; therefore, this mixed population is collectively known as microvesicles (MVs).
Any number of factors could have influenced the initial growth of the tumour cells in vivo that would not have been present in vitro.
Our computer-assisted modeling sheds new light on why we observed a dramatic reduction in PGRN endocytosis when ligands, such as NTS and human PGRN588 593, were present in vitro: the binding pocket and protein protein interactions of NTS and PGRN's C-terminal binding motif significantly overlap, hence explaining the competitive inhibition effect.
This may facilitate the potential removal of non-native Pseudonocardia bacterial strains to prevent within-colony competition between strains, which has been shown to be present in in vitro Petri plate bioassay experiments [56].
Further, we tested whether the in vitro binding of GST-DND1 to APOBEC3 is dependent on RNA or DNA that may be present in the in vitro transcription/translation reactions.
The epigenetic status that underlies some diseases will be erased during somatic cell reprogramming, while for other conditions a suitable phenotype may not be present in an in vitro setting [ 59].
Polyclonal antibodies raised against Bm-iPGM reacted with recombinant Bm-iPGM and with the ES product demonstrating it to be present in the in vitro excretory-secretory products of female B. malayi worms).
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