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In COXPD1 patients, the mutant EF-G1 protein was found to be present at reduced levels in mitochondria of non-affected tissues like the heart but non-detectable in mitochondria of tissues with severely reduced mitochondrial function like the liver [5].
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The most likely candidates for novel MARCH-VIII substrates were CD44 and CD81 since both proteins were present at reduced amounts in all three biological replicas.
Several transcripts such as PR1 and PR2 that show higher levels in AtGRP7-ox indeed are present at reduced levels in the atgrp7-1 T-DNA insertion line [ 4].
Note that all three blots confirm that L259S, V280A, and R384G are present at reduced levels and that N334H can rescue this defect.
Transcripts encoding the plant defensins PDF1.1 and PDF1.2a, RAP2.3/ERF72/AtEBP encoding a member of the ethylene response factor subfamily B2 of AP2-domain transcription factors implicated in PDF1.2a activation [ 37, 38] and the antimicrobial thionin THI2.2 are present at reduced levels in AtGRP7-ox plants.
All three repeats are present at a reduced density in the genome of the closely related species S. mitis.
A representative ProteinChip SELDI MS analysis of NAF demonstrates discrete peaks at 6500 and 8000 Da from three of four tumour bearing women which are not present or are present at a reduced level in NAF from women with normal breast tissue.
However, more thorough investigations based on Western blot analysis indicate that many mutated ABCD1 isoforms with single amino acid substitutions are not completely absent, as initially suggested, but are present at strongly reduced levels, which are not detected by standard immunofluorescence microscopy.
Therefore, another important strength of this study is that both costs of absenteeism and presenteeism (being present at work, but at reduced work performance) were included, whereas earlier studies included only costs of absenteeism [ 15, 16].
To our surprise however both are present at roughly 10-fold reduced levels compared to the β-barrel without epitope due to degradation.
A reduced range of motion was present at the ankle in the transverse plane, together with a reduced sagittal range of motion.
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