Another TEGDI-based PUEs were prepared with either poly(oxypropylene) glycol (PPG) or poly(caprolactone) glycol (PCL).
Six mixes were prepared with either quartzitic gravel or expanded-clay aggregates, and two types of fibers were adopted as well.
Four samples were prepared, ranging from a pure Nd-sialon to a pure Li-sialon, with two intermediate samples being prepared with either lithium or neodymium replacing the other α′-stabilising additive by 20 eq.%, as to maintain an equivalent design composition in all samples.
Swimming plates were prepared with either Bromfield medium (0.04% tryptone, 0.01% yeast extract, and 0.01% CaCl2.2H2O) containing 0.3% Bacto agar or with MM containing 0.3% purified agar.
A total of 100 Eppendorf tubes were prepared with either 65 mg of soil (64.02 ± 2.76 SD) or 65 mg of soil added 10% hoof powder (63.78 ± 2.61SD).
Working DNA solutions were prepared with either 500 mM acetate buffer (pH 4.80) or 20 mM Tris-HCl buffer (pH 7.00), according to the hybridisation protocol [ 38].
A transfection mix was prepared with either 5, 10, or 15 μg of DNA of the STEMCCA vector or control GFP lentiviral vectors (EF1α-GFP; both kindly provided by Dr Gustavo Mostoslavsky), packaging cassette (REV and Gag/Pol, 10 μg) and the VSV-G (5μg) envelope expression cassette, respectively.
To determine whether the RFHVMn LANA contains a functional NLS, EGFP fusion constructs were prepared with either the Region I peptide 24TKRCLPP30 rfI24 300) positionally corresponding to the 24RKRNRSP30 NLS of KSHV, the putative Region II NLS peptide 40ARKRRKG46 rfII40 466), or the entire peptide from Region I to Region II (rfI+II20 48) (see Fig. 1).
Paired sets of proteoliposomes were prepared with either the complete vacuolar mixed lipids (VML) or with 70%PC/30%% PS.
