If that was folly, then Obama, if he's true to his word, is preparing to replicate it.
Three biological replicates were prepared to enable a confident selection of identifiable proteins, and five LC-MS/MS technical replicates were performed to maximize the number of identifiable proteins and the number of spectral counts per protein [ 7, 8].
Replicate plates were prepared to allow sampling at different times, including 24, 48, and 168 h after incubation.
Seven replicate leaf discs were prepared to ensure sufficient material for RNA purification and microscopic examination following infection.
Here, we present similar mitochondrial forms in situ, in a tumor cell that was not preparing to replicate because its nucleus was partly degraded and an early EC and potential megakaryocyte accompanied that cell.
Three independent biological replicates were prepared and each replicate included root materials from three or more of P. ginseng plants.
Practicality: The mirnaTA only requires a simple input file which can be prepared by anyone, takes up to three replicates and 20 time points, and generates publication quality graphics in PNG format.
For each tissue sample, 3 biological replicates were prepared; each biological replicate comprised of RNA extracted from tissues collected from 4 to 5 fishes.
Samples were prepared in replicate and Illumina HumanHT-12 v4 expression arrays were used to detect changes in gene expression.
mRNA was prepared from replicate (>6) cultures on indicated days from undifferentiated hESCs (day 3), d0, d5, d15, d22, and d25 cells.
