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This microprobe can be prepared in batch with good reproducibility and stability, and its motion speed can be conveniently visualized by optical microscope.
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Tetra(4-pyridyl porphyrinato-manganese(III) sulfate [MnIII TPyP)]+ was prepared in batch samples as described in literature [24].
Importantly, sensor substrates are prepared in batch using well-established microspotting techniques.
PCR reactions were prepared in batch using the 2× Master Sybr Green PCR mixes (Applied Biosystems) without primers for each cDNA sample so that comparisons between levels mRNA of the control gene (G6pdh) and the experimental (SpPI) would not be affected by differential cDNA loading errors.
The proposed SERS substrates were prepared in batches for the practical PQ sensing.
Non-supplemented DW medium was prepared in batches of 1 L by mixing 0.5 L whey permeate with the same volume water.
Therefore, the materials were prepared in batches containing GLU in an initial concentration of 50 mmol L−1 together with the background electrolyte 100 mmol L−1 NaClO4.
The samples were prepared in batches, according to manufacturer guidelines, where the sample was added directly to the cleaned resin, stirred for 60 minutes, and then centrifuged to remove the resin from the sample.
The mash for ethanol fermentation was prepared in batches of 1000 g total weight.
Flies were prepared in batches of 12 samples; each batch contained 10 individual flies and 2 sham extractions that served as negative controls.
Diets were prepared in batches as needed and stored in sealed containers at −20°C to prevent bacterial growth and fat oxidation in the food.
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