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Our results show that good quality sequencing libraries can be prepared from small amounts of total RNA and that varying degradation levels in the samples do not have a significant effect on the overall quantification of sncRNAs via NGS.
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(D) Twenty-four hours after PEITC treatment, RNA was prepared from small intestines and RT-qPCR performed to examine intestinal maturation marker gene expression.
Total genomic DNA extracts were prepared from small pieces (≤1 mm) of tissue using the NucleoSpin® 96 Tissue kit (Macherey-Nagel Duren, Germany), following the manufacturer's protocols.
Tissues were paraffin-embedded and 5μm sections were prepared from small intestine and liver.
For sequencing using the Illumina Sequencing by Synthesis system (read length 36 nt), 17 libraries were prepared from small RNAs of seed parts including radical, hypocotyls, cotyledon, embryo, endosperm and seed coat at 15, 25, 35 and 45 DAF using Illumina Small RNA Sample Prep Kit v1.5 following the manufacturer's instructions.
In a firm of any size there is invariably a special department where full-size drawings are prepared from small-scale drawings provided by the designer.
For the first time, PHBs could be prepared from very small loadings of a metallic catalyst (100 ppm) via an "immortal" procedure, thereby allowing the growth of as many as 50 200 PHBs chains per metal center.
High quality sequencing libraries can successfully be prepared from as little as 100 ng small RNA enriched RNA.
Syrup, in small amounts, can be prepared from its sap.
Be prepared from your side.
Hog casings are prepared from stomach, small intestine (smalls), large intestine (middles) and terminal end (colon) of the large intestine (bung).
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