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Sufficient cellular material for Lsd1 ChIP-Seq could not be prepared from primary Gr1dim Macellsells to generate high-quality occupancy data.

Whole cell extract was prepared from primary dermal fibroblasts and keratinocytes.

RNA was prepared from primary myoblast or C2C12 cell cultures using Trizol reagent (Gibco).

Lysates were prepared from primary myeloerythroid cell suspensions from BM and spleen by direct boiling, fractionated by SDS-PAGE, and immunoblotted as previously described [21].

Lysates were prepared from primary MECs isolated from Lrp5 +/+, Lrp5 −/−, and MMTV-Wnt1 mice (to examine the loss- and gain of function conditions).

Whole-cell protein extracts were prepared from primary tumors.

Peripheral monocytes were prepared from primary pSS patients and normal individuals.

Serum-free CM was prepared from primary cultures of chondrocytes or FLS isolated from WT or Epas1+/− mice (C57BL/6).

Single cell suspensions used for MagSweeper tumor cell isolation were prepared from primary and metastatic tissue from breast cancer patients.

Immortalized MEFs (MEFi) were prepared from primary MEFs by sequential passages as for 3T3 cell line establishment.

Formalin-fixed and paraffin-embedded (FFPE) tumour samples were prepared from primary surgical or biopsy specimens in lung.