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For example, an empty product vial can be prelabelled with partial information (e.g., product name, batch number, date) prior to filtration of the radioactive product, and upon completion of the QC test, the outer shielded container can be labelled with the required information (e.g. radioactivity).
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Parasites and THP-1-derived macrophages were prelabelled with CellTrackerTM Green and CellTrackerTM Dil, respectively, and co-incubated at a ratio of 1∶10 (cells∶parasitetes). 16 h post infection, 30 to 70% of the THP-1-derived macrophages were found to be infected.
For intracellular cAMP, cells were prelabelled with tritiated adenosine for 2 h.
In this assay, cells are prelabelled with BrdU for 24 h and BrdU foci are detected under non-denaturing conditions.
Cells in six-well plates were prelabelled with 1 μCi ml−1 myo-[H] inositol for 24 h in inositol-free RPMI 1640 medium containing 0.3% BSA.
Human umbilical vein endothelial cells were prelabelled with a vital, fluorescence dye, 5 μ M 5- and-6 -carboxyfluorescein diacetate, succinimidyl ester (5- and-6 -carboxyfluorescein, Eugene, OR, USA) for 10 min at 37°C.
When time course experiments were performed, the mice were prelabelled with 12.5 μCi Ca and the kinetics of the release of Ca was analysed by withdrawal of small amounts of medium at the stated time points.
Fibroblasts were prelabeled with Hoechst 33342.
Three or two mice bearing tumor of each group were prelabeled with intra-peritoneal injection of BrdU (100 mg/kg, Sigma-Aldrich, Inc., Aaint Louis, MO, USA) 15 hours before sacrificed.
HDFs were prelabeled with Calcein AM as described above.
Cells were prelabeled with CM-DiI before cell injection for tracking in histological sections.
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