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Toxicity data could not be pooled due to differences in reporting adverse events.
These studies cannot be pooled due to different measures of outcome across studies.
These studies could not be pooled due to the use of different comparators.
Data of the five trials could not be pooled due to ambiguous definition of improvement of symptoms.
Data from all four trials could not be pooled due to heterogeneity among them (p = 0.0001; I² = 86%).
These studies investigated quite separate health issues, did not use comparable methodology and data could not be pooled due to their heterogeneity.
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After reverse transcription cDNA was purified using Qiaquick PCR purification kit (Qiagen), according to manufacturer's instructions, and equivalent concentrations of the three different cDNA samples were pooled, due to the lack of sample cDNA.
Levels of mRNA and protein were measured individually except for tadpoles at stages 27 and 28 where >10 animals were pooled due to the small size of tadpoles in the early stages of B. marinus development.
For multivariate analyses, the categories 'moderate' to 'very bad' were pooled due to low cell frequencies.
Locations that were pooled due to sample size limitations are labelled with an asterisk.
Data from all or selected years were pooled due to the small number of cases.
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