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We present a new process for creating highly selective and cost-effective copper patterns that can be plated on virtually any substrate including plastics by combining polyelectrolyte multilayer (PEM) coatings, microcontact printing, and electroless deposition.
Regardless of the enrichment step, the specimens have to be plated on selective media.
The selective medium will be plated on the ESBL chromogenic medium following the same procedure as for direct plating.
These specimens may be plated on screening medium, either directly or after an enrichment step in broth containing imipenem 0.5 1 μg/mL or ertapenem 0.5 μg/mL [ 98, 99].
Briefly, the cortices of newborn (1 or 2-day old) ICR mice pups were aseptically removed, chopped into pieces and trypsinized to be plated on T75 culture flasks (Thermo Fisher Scientific Inc).
These microcapsules could then be plated on regular Petri dishes and incubated on complete media at the permissive temperature to recover the desired strains, either wild type or mutant (data not shown).
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After enzymatic digestion, single cells were plated on a tissue culture dish for one hour.
Cells were plated on SD Trp plates and revertants (regular size colonies) were isolated and lysed.
The intracellular bacteria were plated on TSA plates, incubated at 37 °C overnight and CFU was counted.
The dissociated Ff-hiPSCs were plated on rLN511E8-coated plates at high density (7.5 × 105 cells/cm2; Day 0).
The sorted cells were plated on 96-well plate for single cell colony selection.
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