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Cells should then be placed in selection media [growth medium + puromycin (~1 μg/mL, determined empirically)] and selected for 48 72 h before sorting or dilutional cloning.
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Cells were allowed to recover for 48 hours before being placed in selection medium.
Infected calli were placed in selection medium to allow resistant calli to form.
However, it is not known what confidence could be placed in these signatures of selection if a different set of divergent populations was used, or if intermediate populations were included.
Consequently, when the England team was chosen, he asked for a medium-paced bowler to be placed in reserve in case conditions warranted their selection.
Following transfection, cells were placed in a selection medium containing 0.5 mg ml−1 G418 (Invitrogen).
Cells were placed in puromycin selection media beginning on day 2 post-transfection.
Codons with ω≤0.1 and a statistical significance of p<0.01, adjusted for multiple comparisons, were placed in the "extreme purifying selection" category.
PAML and SLR analyses of the apicomplexa and vertebrate subgroups resulted in slightly more codons being placed in the extreme purifying selection category (112 in the apicomplexa and 121 in the vertebrate clades as opposed to 93 in the protozoan and 97 in the metazoan clades).
To do this, we used a subset of the taxa in each of the two groups (apicomplexa for the protozoan clade and vertebrates for the metazoan clade) and repeated the analyses, which resulted in slightly more codons being placed in the extreme purifying selection category.
After incubation, the cells were diluted 1 3 with PBS/1%HSA and the tubes were placed in a magnet holder for selection of the cell-immunobead rosettes.
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