Sampling took place along six 200 m long transects, whereof three were placed in primary forest and three in secondary forest habitat.
Transdifferentiation of primary acinar cells to ductal cells occurs when the cells are placed in primary culture [14], [15] Acinar-to-ductal transdifferentiation may occur in experimental pancreatitis and in the progression to pancreatic neoplasia [16], [17], [18], [19].
During clinical practice all students were placed in primary health care centres and all had a preceptor assigned.
Fresh neoplastic masses from the specimen were placed in primary culture, and the remaining samples were fixed in 10% formalin for histopathology.
In brief, microneedles containing a chemoattractant are placed in primary tumors while the tumor-bearing mouse is alive and under anesthesia.
Nuclei were transported to our laboratories in vials with gauze moistened with balanced salt solution at room temperature and were placed in primary fixative within 4 hours of extraction.
Coverslips were placed in primary antibody (Table S3) solution for 90 min in 2% GS, washed, then placed in relevant secondary antibody solution (Invitrogen Alexa Fluor-conjugated, in 2% GS, all 1 1,000) for 1 hr, washed, and mounted in MOWIOL (Calbiochem).
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