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Candidate CTCs that are identified after CyteFinder® imaging can be picked using a software module that positions the needle tip over the cell of interest.
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Loci with no missing data in all 10 individuals of Siniperca were picked using a custom Perl scripts (picktaxagene.pl).pl
The best agent is picked using a set of rules from a rule base, which is currently maintained manually by an expert.
The resulting transmitted waveforms were picked using a customized Graphic User Interface (GUI) and then corrected for zero-time and column shell delays.
Cells were picked using a sterile extended pasteur pipette from a dish, washed in fresh autoclaved seawater to check for single isolation under a microscope, photographed and picked using a separate autoclaved, pipette.
Plaques were picked using a sterile Pasteur pipette and transferred into 1 ml SM.
White colonies were picked using a Q-pix automated colony picker (Genetix Ltd.
Briefly, each protein spot on SYPRO Ruby stained gels was picked using a spot picker (Amersham Biosciences).
Subclones from each BAC clone were picked using a QBOT (Genetix, New Milton, UK).
White colonies were picked using a Genetix Q-Bot and stored in 384-well microtiter plates (Genetix) at -80°C.
Individual colonies were picked using a sterile pipette tip and resuspended in 10 μl of PCR mix.
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