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Surprisingly, Y34F mutation did not abolish tyrosine phosphorylation of C7ORF41, suggesting that C7ORF34 might be phosphorylated at multiple tyrosine sites.
The regulation of Hsp90 chaperone activity by phosphorylation is more complicated, as Hsp90 has been shown to be phosphorylated at multiple sites [ 23– 25].
This implies that Synj is likely to be phosphorylated at multiple sites, but the p-Synj antibody may not be able to distinguish between all the sites.
Tau is a phosphoprotein that can be phosphorylated at multiple serine and threonine residues.
At the protein level, MITF is modified post-transcriptionally and has been shown to be phosphorylated at multiple sites leading to either increase in transcriptional activation or decrease in protein stability [14], [15], [16], [17].
This difference in sensitivity to λppase treatment of the two detection methods implied that SIRT1 could be phosphorylated at multiple residues, and could contain phospho-threonine and phospho-tyrosine residues in addition to phospho-serine.
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We show that Puma is phosphorylated at multiple sites, with the major site of phosphorylation being serine 10.
Phospho-peptide mapping revealed that XopE2 is phosphorylated at multiple residues in planta and residues T66, T131 and S334 are required for maximal binding to TFT10.
Biochemical studies establish that the SNS cytodomain is phosphorylated at multiple tyrosines and their site-directed mutagenesis compromises the ability of the corresponding transgenes to rescue myoblast fusion.
Staining of adjacent sections with p409-410 (Fig. 2o, p) and another antibody that recognize the phospho-epitope Ser403/Ser404 (Fig. 2q, r) revealed that TDP-43 aggregates were phosphorylated at multiple sites.
IRS1 is the principal IRS protein and is phosphorylated at multiple tyrosine sites upon insulin stimulation (Sun et al., 1993).
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be phosphorylated at certain
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