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Polymerase chain reaction will be performed with specific primers at concentrations of 900 nM.
These exercises can be performed with specific apparatus (Equipment-based Pilates) or without them (Mat Pilates) [ 11].
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qRT-PCR was performed with specific primers for each genes.
Kinetic enzyme assays were performed with specific chromogenic substrates (Agrawal 2004).
Stainings were performed with specific antibodies and fluorescently labeled secondary antibodies.
Polymerase chain reactions were performed with specific primers using the Taq polymerase kit (Eurobio).
After first strand synthesis, polymerase chain reaction (PCR) was performed with specific intron spanning primers.
PCR was performed with specific primers for unc-18 using GoTaq DNA polymerase (Promega).
Briefly, to detect mature miRNA species, reverse transcription and amplification were performed with specific primers and TaqMan probes.
Immunoblotting was performed with specific antibodies for cleaved caspase-3 (9664 cell signaling), FoxM1 (kind gift from Dr. Costa's lab) and β-actin (A5441, Sigma).
Amplification of the BxeA3900 gene (hmgA2) was performed with specific primers HmgA2-f (5'-TACGCCGAACACTGTCAGTTCG-3') and HmgA2-r (5'-GCCCCGAAATTCTCGCAGATGTA-3').
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