Experiments were performed with minimum six different donors (three different donors per group).
Although, more informal analyses can be performed with a minimum of n = 30 per subgroup [ 28], more than 200 participants per subgroup is generally recommended [ 50].
At least 30 sentinel node procedures followed by an ALND of at least level I and level II have to be performed, with a minimum of 27 patients with accurate sentinel node identification.
The scoring method we used consists of simple definitions, is reproducible, can be performed with a minimum of additional reviewing time and can be applied to a whole range of different CT protocols (including non-gated CT).
The 18F Fluoride PET/CT procedure could be performed with 2.9 MBq/Kg (minimum 185 MBq, recommended at least 200 MBq) of 18F NaF to minimize the effective radiation dose received, maintaining the quality of the scan.
The use of amplified nucleic acids means that for the first time full characterization of hESC lines can be performed with little time investment and a minimum of material.
Intracellular cytokine staining was performed to determine whether the response was mediated by CD4+ or CD8+ T cells, shorter peptides were used to map the minimum epitope, and peptide titrations were performed with the minimum epitope to determine the functional avidity of the response (peptide concentration giving half-maximal response).
Assemblies were performed with a minimum percent identity score of 98, a minimum overlap length of 500 nt, a match score of 1, a mismatch penalty of -2, a gap penalty of -2, and an additional first gap penalty of -3.
While sweets, sweets, sweets were the draw there, the reaching -- as well as the other balletic exercises -- was performed with a minimum of complaints.
