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As suggested by Peeters et al (2011) we believe that these studies need to be performed with control series in the context of randomised trials.
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The SSH analysis was performed with control samples as the driver and heat-stressed samples as the tester.
Similar experiments were performed with control HepG2 cells exposed to Al for 24 h and Al-treated HepG2 cells incubated in 5mM KG for 24 h.
Stimulation was performed with control medium, highly purified lipopolysaccharide (Escherichia colli 055 B5, sigma, extra purified according to Hirshfeld et al. [29]) at various concentrations (1, 10 and 100 ng/ml), and the lipopeptide Pam3Cys (10 µg/ml, EMC Microcollections, Erlaugen).
Immunopurification was performed with control IgG or anti-CEACAM5.
Immunopurification was performed with control IgG or anti-CTB.
Stimulation was performed with control medium or 100 ng/ml EGF medium.
RNA extraction was performed with control and stimulated RA synoviocytes before and 20 min after cytokine addition.
The microbalance was calibrated using National Institute of Standards and Technology standard weights, and balance checks were performed with control filters after every five filter weights.
ChIP was performed with control, anti-WT1 or anti-BASP1 antibodies using fragmented cross-linked chromatin prepared from either V-K562 or B-K562 cells.
Raw data were intra-normalized with the mean signal intensity of the ACTB gene after subtracting the mean intensities of the negative control spots (pUC18 plasmid DNA and blank spots. Inter-normalization was performed with control normal bone signal intensities.
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