In view of the latter, molecular analysis of the PLOD1 gene was performed, which excluded its contribution to the phenotype.
However, a significant ERD could still be observed following TMS at 85% RMT over both ipsilateral and contralateral motor areas, in the two subjects so tested, and here simultaneous EMG recordings were performed, which excluded any muscle responses.
A minimal diagnostic evaluation to exclude other diseases was performed, which consisted of complete blood cell (CBC) count, serum chemistry, fecal examination, survey radiography, and abdominal ultrasonography.
To exclude dysganglionosis, a rectal biopsy was performed, which yielded normal findings.
After excluding hypothyroidism, a barium enema was performed which revealed an obstruction at the splenic flexure of the colon.
Because of the chemically undefined character of embryonic tissue homogenates, and the possible inclusion of soluble and particulate derivatives of embryonic cartilage anlage [ 24], which possess morphogenetic action, our experiments are performed with media which exclude embryonic extracts.
To investigate the extent to which this may have influenced our results, a sensitivity analysis was performed in which we excluded all lab tests carried out in individuals diagnosed with diabetes in the same year (incident cases).
The absolute value differed in the several experiments, which were performed to exclude artifacts.
Glucose-insulin (40 mL of 50% glucose containing 8 units of insulin) was infused simultaneously with another ABG analysis, which was performed to exclude mechanical failure (Table 1, T3).
Gastroscopy, which was performed to exclude esophageal mucosal involvement that might be responsible for dysphagia that proved normal.
