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The assessment itself is a significant task and should be performed using validated instruments.
Analysis of samples will be performed using validated liquid chromatography tandem mass-spectrometry.
Therefore, an ideal assessment of symptoms and HRQoL should be performed using validated patient-reported outcome (PRO) instruments [ 2].
Where FFR cannot be performed due to clinical/safety reasons, quantitative coronary angiography (QCA) will be performed using validated commercial vendor software.
Analysis of the 5 ml urine aliquots for antibiotic clearance will be performed using validated LC-MS/MS assays and will determine the rate and amount of antibiotic excreted in urine.
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Analyses of stool samples collected during GE episodes were performed using validated procedures in a GSK laboratory or GSK validated laboratory.
Analysis of plasma samples was performed using validated methods based on solid-phase extraction followed by high-performance liquid chromatography tandem mass spectrometry.
Measurements of DTG concentrations were performed using validated analytic methods based on protein precipitation, followed by high-performance liquid chromatography tandem mass spectrometry analysis [ 21].
A broad range of endocrine tests are performed using validated immunoassay technologies and methods including radioimmunoassay, enzyme-linked immunosorbent assay and chemiluminescent immunoassay.
Quantitative reverse transcriptase PCR (RT-PCR) was performed using validated TaqMan Assays (Applied Biosystems) on an Applied Biosystems 7900HT PCR system and expression normalized relative to GAPDH as described [23].
The quantification of selected four marker compounds was performed using validated HPLC method [ 6].
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CEO of Professional Science Editing for Scientists @ prosciediting.com