Sentence examples for be performed using radiolabeled from inspiring English sources

Visualization of in vivo reporter gene expression can be performed using radiolabeled substrates, antibodies, or ligands.

Analysis of modified EGF receptor ligand-binding capability was performed using radiolabeled [125I]EGF (PerkinElmer), exactly as described [16].

Further biodistribution studies were performed using radiolabeled fusion proteins.

Southern blot analysis [ 29] of genomic DNA was performed using radiolabeled 522 bp annexin I cDNA product from the PCR reaction as described above.

To investigate the ability of IntI1 to interact with the target sites attI1 and attC, standard gel mobility shift assays were performed using two radiolabeled fragments containing either the double-stranded attI1 or the attC site (respectively attI1ds and attCds).

Northern blot analysis was performed using a radiolabeled probe to detect the small RNA of interest.

To test whether the lamprey ER and the amphioxus ER are able to bind DNA on specific estrogen response elements (EREs), electrophoresis mobility shift assays were performed using a radiolabeled consensus ERE sequence (see Additional file 2).

To determine whether c-Myc was able to bind to any of these putative E-boxes, a series of electrophoretic mobility shift assays were performed using double-stranded, radiolabeled oligonucleotides that encompass each of these sites or a known Myc consensus site from the carbamoyl phosphate synthetase-aspartate transcarbamylase-dihydroorotase (CAD) promoter.

Quality control of the radiolabeled PSMA ligands was performed using high-performance liquid chromatography (HPLC) with a C-18 reversed-phase column (XterraTM MS, C18, 5 μm, 150 × 4.6 mm; waters) (Additional file 1).

The RNA was isolated and then primer extension was performed using AMV reverse transcriptase and a radiolabeled DNA primer to the 100 120 region or to the 70 90 region.

In vitro synthesis of radiolabeled proteins was performed using the TNT® coupled reticulocyte lysate system (Promega) and S methionine.