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We show that their encapsulation may be performed using methods typically used for hydrophobic molecules and that absolute lipophilicity is not necessarily a prerequisite for encapsulation into a hydrophobic carrier such as polystyrene.
Molecular genotyping and sub-genotyping of M. leprae can be performed using methods based on SNPs.
As a last resort, pooling can be performed, using methods that are based on a random effect model.
Equality comparisons of scalar values such as numbers or dates can also be performed using methods similar to those of Protocol 2 – the scalar value is treated as a sequence of bytes and is transformed by a keyed hash algorithm in the same way as a character string.
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Genomic DNA isolation from leaves was performed using methods similar to those previously described (Okamura et al. 2013).
MDP eQTL mapping was performed using methods that have been previously described [54].
All samples were kept frozen, and DNA extractions were performed using methods described elsewhere [34].
Calcium imaging based on intensity or ratio measurements was performed using methods described previously [28].
DNA sequencing was performed using methods and primers as previously described.
2DGE and silver staining of the resulting gels were performed using methods previously published[16] (see Methods S1).
Retinotopic mapping and functional mapping of area MT+ was performed using methods identical to those described previously [19], [21].
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