Gene ontology analysis was performed using Functional Annotation Clustering as part of DAVID [74].
Gene functional annotation was performed using functional classification tools such as David Bioinformatics Resources and Ingenuity Pathways Analysis web-software.
The pathway analysis was performed using Functional Ontology Enrichment/ Pathways Maps default option in MetaCore for mouse or human depending whether mouse or human data were analysed, respectively.
After filtering for non-redundant, intronic, exonic and promoter model matches within gene loci of annotated genes, knowledge-based pathway analysis was performed using functional co-citation settings.
Each analysis was performed using the functional annotation charting and functional annotation clustering options.
Functional clustering was performed using the functional annotation clustering tool available on the Database for Annotation, Visualization and Integrated Discovery (DAVID) online software from the National Institute of Allergy and Infectious Diseases [ 13].
The functional enrichment analysis was performed using the functional annotation tools of DAVID (the Database for Annotation, Visualisation and Integrated Discovery [ 31]) to determine if zinc deficiency resulted in regulation of genes of any particular type or function.
Gene ontology analyses were performed using the functional annotation clustering function of DAVID (http://david.abcc.ncifcrf.gov/).abcc.ncifcrf.gov/
A comparative theoretical study of three benzimidazole isomers, benzimidazole (BI), 2-methylbenzimidazole (2-CH3-BI), and 2-mercaptobenzimidazole (2-SH-BI), as corrosion inhibitors was performed using density functional theory (DFT) with the B3LYP functional basis set.
