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EGFRvIII analysis alternatively can be performed by reverse transcription-PCR analysis using primers located in exons 1 and 9 [ 79].
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The inverse LWT can be performed by reversing the prediction and update operators and changing each '+' into '-' and vice versa.
The I V measurements were performed by reverse scan (RS) and forward scan (FS).
Usual Affymetrix small-mass RNA amplification to incorporate fluorescent probe into the plasma-derived RNA for subsequent Affymetrix Gene 1.0 ST microarray was performed by reverse transcription followed by two rounds of in vitro transcription with T7 RNA polymerase and final regeneration of fluorescently labeled cDNA.
HCV genotyping was performed by reverse hybridization (Inno Lipa HCV, Innogenetics, Gent, Belgium).
Total RNAs were cleaned using DNase 1 kit (Cat. No: 18068-015, Invitrogen), and the first-strand cDNA synthesis was performed by reverse transcription of mRNA using Oligo dT 20 primer and SuperScript™ III Reverse Transcriptase (Cat. No: 18080-051, Invitrogen).
Combined spectroscopic, radiographic, and chromatographic characterization of the CNT construct was performed by reverse phase HPLC, using a Beckman Coulter System Bioessential 125/168 diode array detection instrument equipped with an in-line γ-RAM Model 3 radioactivity detector (IN/US, Tampa, FL).
Total RNA (1 μg) was performed by reverse transcription (RT) using reverse transcription kit (Toyobo, Japan).
The negative control was performed by reverse transcription in absence of primers followed by PCR amplification.
Knockdowns by siRNA were performed by reverse transfection at 25 nM final concentration of siRNA.
Genotyping was performed by reverse hybridization, and viruses were not sequenced; misclassification could have occurred [ 43].
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