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Primary rat Schwann cells can be passaged indefinitely in culture while maintaining normal cell-cycle checkpoints [4].
It was of interest to note that supernatants of cells co-infected with these two defective viruses were infectious and could be passaged indefinitely in the absence of VSV G trans-complementation.
Although Nanog−/− iPSCs could not be established in 2i+LIF, once naive pluripotency was established in chiron+LIF, Nanog−/− iPSCs could be passaged indefinitely in 2i+LIF, consistent with known discrepancies between NANOG requirement in pluripotency establishment versus maintenance.
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These hiNSCs can be passaged indefinitely and cryopreserved as colonies.
Note, however, that mammospheres formed from CD44-negative populations were unable to passage and generate secondary mammospheres whereas CD44+ mammospheres could be passaged indefinitely.
In addition, L929 fibroblasts are recommended by ISO 10993-5 for cytotoxicity tests [ 21], and HaCat cells are suitable substitutes for oral keratinocytes because they can be easily grown and passaged indefinitely [ 22].
Now Russia will be frozen indefinitely in daylight saving time.
These cells can be maintained indefinitely in culture and yet maintain competence to produce all the cells within a fetus.
Because of the high frequency of genomic mutations during long-term passaging of ESCs and iPSCs, it is necessary to determine whether hUC-MSCs, such as ESCs and iPSCs, can be grown indefinitely in culture and undergo adaptive genetic changes during the long-term expansion.
Parliamentary elections were postponed indefinitely in March.
The NPT was extended indefinitely in 1995.
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