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Exact(1)
This can be overcome by constructing a smaller informative microsatellite marker database comprising markers located in potentially functional genic sequences with relatively high polymorphic potential.
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This limitation may be overcome by a sweeping antibody construct that has both pH-dependent antigen binding and increased binding to cell surface neonatal Fc receptor.
This can be overcome by directing the transposon construct to a targeted site using the Gal4/UAS or FLP/FRT system [ 40, 42, 43], or by surrounding the transgene with an insulator [ 44].
We had previously observed that expression of fluorescent tagged RPGRIP1α1 alone produced RPGRIP1α1 aggregates (unpublished results), a phenomenon that could be overcome by lowering the expression of RPGRIP1α1 from constructs lacking poly-adenylation signals to promote mRNA decay (Lu et al., 2005).
The goal of achieving saturation for libraries constructed from a specific tissue may be overcome by combining library information available in public domain databases, if those resources are large enough.
These problems seemed to be overcome by two independent studies in which pronuclear-delivered shRNA constructs were successfully used to knockdown genes in mice [7], [8].
This limitation can only be overcome by developing new behavioral tests for non-human animals that have predictive, construct, and etiological validity.
This limitation can be overcome by modifying the standard Poincaré plot to include the relative frequency of pairs of consecutive data points by constructing a 2D histogramc of RR(n), RR(n + 1).
Fragility could be overcome by toughness.
This can be overcome by RFID technology.
This could be overcome by tissue engineering.
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