FPKM parameters K and M are optimized to individual experiments in the software used to assemble the transcriptomes, in this case Cufflinks [ 66], was used (for more detail, refer to [ 64]).
From linear regression analysis the dosage ratio of individual herbs could be optimized to maintain same component contents with traditional decoction as much as possible.
Scale construction could be optimized to take into account the impact of anticipated group differences or individual changes in appraisal.
These parameters can be optimized to improve the predictions.
RT-PCR amplification parameters were optimized to analyze individual target genes and gene specific primers were used.
Analysis parameters for images acquired by Celigo Imaging Cell Cytometer were optimized to identify individual MEF and COS cells based on fluorescence, and the average green and red fluorescence intensity per cell was determined at the different time points assessed.
In this regard, the study design is optimized to decipher the individual role of VDR activation, and the effect of combining VDR activation with conventional RAS inhibition, since this combination therapy has been shown to provide synergistic renoprotection in animal models of diabetes [ 19- 21].
The thicknesses of the individual layers were optimized to achieve high solar absorptance, while the colors were designed with the TFCalc optical design software.
This eligibility criterion was optimized to look for evidence of HIV superinfection; hence, individuals with only negative or unknown-status partners were excluded.
The individual gains of each filter were optimized to achieve a quasiflat frequency response.
