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First of all, the electrochemical conversion of the kinase inhibitors had to be optimized for substrate conversion and product formation.
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Thus, the labeling procedure has to be optimized for each protein and fluorescent substrate separately concerning substrate concentration and incubation time.
The acceptor subsites of GH70 enzymes seem thus to be optimized for recognizing and utilizing the products made by the enzyme as acceptor substrates in a polymerization reaction.
Some will be optimized for voice.
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The assay was optimized for temperature, substrate concentration, size and volume proportions of the immuno-sorbent matrix, enrichment and reaction times.
Therefore, ferritin active sites are optimized for iron substrate access, which is consistent with the hydrophilic protein environment near the biferrous site.
Process parameters were optimized for Si substrates and transfer of the process to ceramic LST/YSZ substrates is possible in principle.
Substrates were optimized for each kinase.
The substrate concentration was optimized for each enzyme to achieve first-order reaction kinetics.
If specific and functional kinase- or phosphatase-substrate interactions are optimized for binding compared to promiscuous interactions, then changes in phosphorylation should occur faster on functional versus promiscuous substrates.
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