Sentence examples for be optimized for expression from inspiring English sources
Many amino acids are needed to be optimized for expression in human cells (more than 79%) (see Supplementary Material available online at http://dx.doi.org/10.1155/2013/384091).org/10.1155/2013/384091
To facilitate these studies, the receptor had to be optimized for expression in Xenopus oocytes by altering the signal peptide and by introducing a mutation in the pore-lining region.
Gene synthesis is a convenient method of obtaining sequence-verified cloned DNA, especially when the source biological material is not readily available (rare, lost or dangerous specimens), when codons need to be optimized for expression in a particular host or when the desired sequence is chimeric or designed de novo.
The obtained DNA sequences were optimized for expression in E. coli and C. glutamicum by check for RNA secondary structure with software UNAFold (Markham and Zuker 2008).
We achieved a significant increase in soluble p38α protein expression by using the genetically modified E. coli strain BL21 DE3 Rosetta, which is optimized for expression of eukaryotic proteins with codons rarely used in E. coli.
To simplify genetic manipulation and generate p66/p51 heterodimers in Escherichia coli, a gene construct of the viral protease alone was optimized for expression from a separate vector carrying a p15A origin of replication.
All sequences were optimized for expression in yeast by adapting to the yeast codon-usage, contained a C-terminal c-myc-tag, and HindIII-EcoRI sites for subcloning into pYES2.
One bifunctional reporter, which is designed for use in CHO cells, loach, and chicken embryos, combines full length LacZ and GFP coding sequences that are optimized for expression in humans [16].
The different HA genes were placed downstream of a strong vaccinia early/late promoter [17] and were optimized for expression by vaccinia virus removing internal RNA transcription stop signals [18].
The synthesized sequences were optimized for expression in mammalian cell lines.
The codon usage was optimized for expression in E. coli [see Additional file 1].
