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Once the model matrices are derived for a certain WT type they need to be optimized for each individual turbine of this type with respect to the first eigenfrequency Ω 0. The general optimization procedure is described in [4].
Therefore, the time had to be optimized for each type of graphitic structures by using different times (10, 30, and 50 min) while the other parameters were kept constant (at 1050 °C under N2/H2/C2H2 flow rates for 50/10/50 sccm) to determine the effects of the reaction time on the graphitization, as well as the morphology of the products.
Taken together, RT-qPCR quantification requires the simultaneous measurement of a panel of candidate reference genes rather than few empirically-determined or pre-validated reference genes and that robust data normalization needs to be optimized for each particular assay.
Exact conditions need to be optimized for each tissue type and targeted application.
Our experience has been that conditions need to be optimized for each laboratory.
PCR includes a series of well-known steps that need to be optimized for each specific application.
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The collision energy was optimized for each lipid class.
The beam pattern is optimized for each recipe.
Cycling conditions were optimized for each PCR 1 product using Q5 polymerase.
Transfection efficiency and conditions were optimized for each cell line used.
The spray process parameters were optimized for each plasma set-up individually.
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