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For example, the surface of the polymer gel can be optimized for cell attachment.
The lifetime of the epithelium might be shorter under BAK conditions whereby not all of the parameters can be optimized for cell performance.
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The array is a multiplex fluorescent sandwich ELISA that contains internal normalization, standards for quantification, and is optimized for cell culture supernatants.
Using a proposed model to describe the cell behaviour based on a 1-D approach, the contacted fraction of the rear contact was optimized for cells with and without BSF.
However, many of these assays can be performed only on living cells and often are optimized for cells grown in tissue culture [ 20].
In situ electroporation of monolayer cell cultures was performed as described before, according to a procedure that was optimized for cell-death studies.
Interestingly, sample and slide preparation were the same for all cell lines tested and only the number of cells and time of transfection had to be optimized for each cell type.
With dependence on the cell type of interest, experimental parameters will need to be optimized for capturing adherent cells, bearing in mind that flow velocity regions scale appropriately.
Many amino acids are needed to be optimized for expression in human cells (more than 79%) (see Supplementary Material available online at http://dx.doi.org/10.1155/2013/384091).org/10.1155/2013/384091
The results provide sufficient evidence that the heterologous siRNA was quite effectively introduced into exosomes using electroporation, but the method may need to be optimized for each exosome and each cell type.
Catalysts in commercial proton exchange membrane fuel cells must be optimized for activity and cost, but improvements are hampered for Pt nanoparticles by low activity resulting from extensive OH adsorption.
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