I was operating with parameters that were very, very tight, with the basic thought in mind that I had better not foul it up". So where, the comic is asked, is the pleasure in it?
A minimum flow rate of 122 mL/min is required to balance the zones in the case of inlet oxygen tension being 110 mm Hg if the BAL is operated with the parameters of Table I.
Combined systems are operated with the following parameters – dual-high power impulse magnetron sputtering (fH = 100 Hz, duty cycle 1%) and dual mid-frequency discharge (fM = 94 kHz, duty cycle 30%) – running simultaneously with two magnetron guns.
The system was operated with the following parameters: electron voltage 70 eV, source temperature 200°C, interface temperature 350°C and acquisition delay for 120 s.
Peptides were injected into the mass spectrometer using 10 µm SilicaTip electrospray emitters (New Objective Cat. No. FS360-20-10-N-20-C12), and the ion source was operated with the following parameters: ISVF = 2500; GS1 = 12; CUR = 25.
The LTQ linear-ion trap was operated with the following parameters: precursor activation time 30 ms and activation Q at 0.25; collision energy was set at 35%; dynamic exclusion width was set at low mass of 0.1 Da and high mass at 2.1 Da with one repeat count and duration of 10 s.
The LTQ linear ion trap was operated with the following parameters: precursor activation time 30 ms and activation Q at 0.25; collision energy at 35%; dynamic exclusion at low mass of 0.1 Da and high mass at 2.1 Da with one repeat count and 10 s duration.
The results show that both A/C systems could be operated independently with different working parameters and operation models.
The mass spectrometer was operated in positive ion mode, with instrument parameters optimized for maximal sensitivity (Supporting Information, Table S3).
The mass spectrometer was operated in the sensitive mode with the following parameters: capillary voltage 1400 V; end plate offset, 500 V; dry gas, 10.0 l/min; dry temperature, 160°C; aimed ion charge control 150000; maximal fill-time 500 ms. The nano-ESI source (Bruker Daltonics, Germany) was equipped with distal coated SilicaTips (FS360-20-10-D; New Objective).
