The Orbitrap Elite was operated essentially as previously described [ 62] in a HCD top 10 mode with dynamic selection of the 10 most intense peaks of each survey scan (300-1750Th) fragmentationtion.
M30 Apoptosense and M65 ELISA kits were both obtained from PEVIVA AB (Bromma, Sweden) and were operated essentially according to the manufacturer's instructions (http://www.peviva.com), as previously described in detail (Cummings et al, 2005; Cummings et al, 2006; Greystoke et al, 2008).
For SWATH-MS data acquisition, the same mass spectrometer and LC-MS/MS setup was operated essentially as described before (Collins et al., 2013; Rosenberger et al., 2014) using 32 windows of 25 Da effective isolation width (with an additional 1 Da overlap on the left side of the window) and with a dwell time of 100 ms to cover the mass range of 400 1200 m/z in 3.3 s.
Even though it has been operating essentially hand to mouth, the institution has had no formal fund-raising operation in place, aside from its annual spring gala.
The Chinese company has been operating essentially without any input from Danone for months because Danone does not have a single executive at the joint venture, despite owning a majority stake.
This drone isn't like most consumer-grade drones for two reasons: firstly, it has a top speed of nearly 70 mph; secondly, it can be operated with a headset that essentially gives you a bird's-eye view of the world, seen through the Vendetta's forward and bottom-facing cameras.
The program was essentially going to be operated by mortgage servicers, who were not prepared to handle millions of modifications.
The DTFB was operated at essentially zero liquid velocity and superficial gas velocities up to 250 m/h.
The two settlers had the same dimensions and were operated under essentially equivalent conditions but differed in terms of being assessed before and after routine maintenance, with the internal 'furniture' configuration of the latter also being modified.
The logistical challenges are even more complicated because during the pop-up swap days, the owners of the two restaurants don't plan to shut down their home bases in Chicago and New York, which means they'll essentially be operating the same restaurant in two locations at the same time.
