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A greater proportion of IgG2 that may be of lower avidity and/or that binds complement less effectively may also offer an explanation for the reduced activity.
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Finally, peptide titration experiments with IL-2 rescued T cells indicated that they were of lower avidity than non-tolerant control T cells expressing the same TCR.
This suggests that one of the bands may be a ligand of higher avidity while the second one is of lower avidity.
Therefore, despite expressing the same transgenic TCR, the cytokine rescued T cells were of lower functional avidity than the non-tolerant control T cells.
Second, studies conducted in the early 1970s [ 72- 74] revealed that antibodies produced by aged as compared with young mice in response to antigenic challenge were of lower affinity and avidity.
This data shows that fucosylated ligands are lower avidity ligands of CT.
However, the antibodies have been found to be of low avidity [ 16].
The IgG1 IgG2 mean ratios showed a significant decrease in all 3 groups over this time period, and the reduction in rSBA GMTs may in fact be due to the greater proportion of lower-avidity IgG2.
IgG was of low avidity (4%, cutoff 15%), indicating acute primary HBoV infection.
More recently, Cerny and colleagues [ 75] have extended these observations by demonstrating that antibodies produced by aged mice immunized with phosphorylcholine immunogens are not only of lower affinity and avidity but are also less protective against infection than those produced by young mice.
The new avidity method permits a meaningful comparison of VZV IgG avidity in serum and CSF and should be of general applicability for easy determination of avidity results, which are not affected by the concentration of specific IgG.
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