Since Sp_Hsp104 provides thermotolerance to both S. pombe and S. cerevisiae, and is also able to reactivate bacterial luciferase, a protein without any close homolog in yeasts, these experiments indicate that Sp_Hsp104 is a disaggregase of broad specificity, as was shown for Sc_Hsp104 [2].
Pancreatic elastase secreted into the GI tract is a protease of broad specificity and is activated in the duodenum by trypsin.
A common problem has been the use of broad specificity reagents such as the antibody clone, RB6-8C5, thegeting the Gr-1 antigen, which recognizes both Ly6G and Ly6C and therefore stains PMNs and monocytes [ 35].
Trypsin is a serine protease of broad specificity that is normally activated in the duodenum to serve as a nutrient protease and as the primary activator of other pancreatic digestive enzymes.
Whilst a potential role for the protein phosphatase family of signalling enzymes in mast cell function has been accepted for some time, the evidence has largely been derived from the use of broad specificity pharmacological inhibitors and results often depend upon the experimental conditions, leading to conflicting views.
Though the study utilized archived samples taken during the 1994/1995 malaria season, the findings have direct relevance for the assessment of naturally acquired antibodies of broad specificity since titres are measured against randomly selected (allelic) antigens and the exact circulating strains/antigenic alleles within study populations are usually not taken into account.
Reviewer 2 A problem, which the authors touch upon, is that of broad-specificity enzymes, such as alcohol dehydrogenase (EC 1.1.1.1) and monoamine oxidase (EC 1.4.3.4), where the reaction is described in general terms, with little no indication of all the substrates that may be involved.
Specific transporter systems for solute uptake are present on both the apical (luminal) and basal (abluminal) membranes and efflux transporters of broader specificity are also present.
However, we have shown that HDA6 is a broad specificity histone deacetylase capable of removing acetyl groups from multiple lysines of multiple histones [16], suggesting that HDA6 might erase histone acetylation throughout the regions to which it is recruited.
