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Sequence alignments were obtained using ESPript [40].

Images were obtained using Chimera [27].

A final assembly was obtained using AMOScmp.

Conservation profiles were obtained using GeneDoc [21].

Similar results were obtained using HeLa cells.

Images were obtained using a 60× objective.

Transmembrane recordings were obtained using standard methods.

Images were obtained using PyMol [52].

Expression values were obtained using gcrma algorithm.

Sequence alignments were obtained using CLUSTALX v1.81 [65].