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(Significant amounts of active full-length protein could not be obtained upon expression in bacteria, consistent with work from Hirokawa on KIF1A motor protein [ Nitta et al., 2004].) Surprisingly, deletion of the CBD in the context of full-length KIF1C did not abolish binding to GST-Rab6A.
Similar(59)
Similar results were obtained upon expression of Sp3 transcription factor (not shown).
Similar results were obtained upon expression of a recombinant fragment of the ErbB2/HER2 receptor protein or GST as MBP fusions.
Similar results were obtained upon expression of a fragment of the ErbB2/HER2 receptor protein or GST as MBP-furS fusions.
Similar results were obtained upon expression of a recombinant fragment of the ErbB2/HER2 receptor protein or GST as MBP fusions, indicating that this approach can generally be applied to enhance secretion and yields of functionally active recombinant proteins.
Transgene expression was verified by fluorescence microscopy and immunoblot analysis, and similar results were obtained upon expression of murine survivin (data not shown).
The WT expression level was set at 1. Sequences of primer pairs used can be obtained upon request.
(Information of primer sequences can be obtained upon request).
Raw data can be obtained upon request.
Primer information can be obtained upon request.
Details can be obtained upon request.
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