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Frozen sections can be subjected to LCM, and enough RNA should be obtained to avoid the amplification step.
Furthermore, subsequent intravascular imaging should be obtained to avoid incomplete scaffold apposition.
This allowed sufficient cell density to be obtained to avoid the ill effects of low density observed for the E populations.
According to these basics and to the more recent studies [ 26, 49], the early repair of the mucin layer by intravesical administration of mucopolysaccarides should be obtained to avoid the chronic evolution of the bladder inflammatory pathologies.
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Finally, the map of safe zone is obtained to avoid component malfunctions and system performance deterioration.
On the basis of the new concept of QSMC, continuous control is obtained, to avoid the chattering phenomenon.
Steady images of at least 20 s were obtained to avoid pressure artifacts, by means of a portable computer and an ADVC110 analog-to-digital video converser (Canopus Co, San Jose, CA, USA).
Between two stimulations, at least a 30-sec pause was obtained to avoid superposition.
Samples were incubated in 5% serum of the species from which the secondary antibody was obtained to avoid non-specific binding.
Hume allows that, speaking imprecisely, we often say a passion is unreasonable because it arises in response to a mistaken judgment or opinion, either that something (a source of pleasure or uneasiness) exists, or that it may be obtained or avoided by a certain means.
The value of the total acid capacity can be obtained avoiding the pre-treatment of the material, and as a consequence the contamination of the sample with other types of electrolytes that can affect the results of the conductometric titration technique.
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