Milligram amounts of construct 4 NA per litre of insect cell culture were obtained from large scale expressions without obvious destabilization of the NA tetramer (based on size exclusion chromatography) or breakdown products (based on SDS-PAGE and western blot) as has previously been the case[12], [15], [16].
To maximize the knowledge that can be obtained from large-scale genomic and proteomic experiments, focus is now being placed on systems biology approaches in which datasets are combined to learn more information than can be gathered from any one dataset alone.
To facilitate reliable design of such structures, pressure data were obtained from large-scale tests conducted in the Jules Verne climatic wind tunnel at CSTB, Nantes, in November 1999.
This paper provides new evidence on international productivity gaps; this evidence is obtained from large-scale firm-level data from the French and Japanese manufacturing industries using non-parametric methodologies designed to overcome confidentiality restrictions.
Fresh dairy manure (DM), chicken manure (CM) and pig manure (PM) were obtained from large-scale farms in Binhai New District and Ninghe District, Tianjin, China.
We base our model on data that are obtained from large-scale studies of IgG substitution therapies [15].
Additionally, 74% (296 452) were obtained from large-scale experiments identifying >100 associations, and hence considered high throughput (16).
Gene essentiality data were obtained from large-scale shRNA screening against 29 BrCa, 28 PaCa and 15 OvCa cell lines [ 2].
Insight into the activation of these processes was obtained from large-scale gene expression analyses in livers of infants at the time of diagnosis [ 4] and from extrahepatic bile ducts and gallbladder (EHBDs) obtained from neonatal mice in a well-established model of rotavirus-induced experimental biliary atresia [ 5].
Stocks were obtained from large-scale preparations in HEK293 cells by double cesium chloride gradients and were quantified by plaque assay (final titers: Ad β-gal = 5.56 × 10 pfu/μl, Ad IL-1 = 3.85 × 10 pfu/μl, Ad IL-1ra = 1.75 × 10 pfu/μl).
