Three short-axis slices of myocardial tissue were obtained and embedded in paraffin.
Explants were embedded in optical cutting temperature (OCT) and snap-frozen in liquid nitrogen prior to cryosection; and 10-μm sections were obtained and embedded on glass slides, fixed with 4% paraformaldehyde (diluted with PBS) for 30 minutes at 4°C, followed by washing three times with PBS for 5 minutes.
Two core-needle biopsies (1.0 mm diameter) from tumor representative areas of each HL case were obtained and then re-embedded in an array master block using techniques originally developed by Kononen et al. [ 29] and then modified by Hedvat et al. [ 30] and Beecher Instruments (Sun Prairie, WI, USA) arraying device.
For some deceased individuals, archived formalin-fixed paraffin embedded tissue was obtained and directly sequenced for the mutation.
In each case, surrogates binding to these targets were obtained and found to contain partner information embedded in their amino acid sequences.
Paraffin embedded tumor material was obtained and processed into a tissue microarray for 549 patients aged less than 40 years (Table 1).
After paraffin embedding, 5-μm sections were obtained and stained with hematoxylin-eosin (H&E).
A linearized model is therefore obtained and embedded into a performance function that measures the tracking error of the output probability density function with respect to a given distribution.
This paper details covercrete sensor arrangements; format of data presentation and information that can be obtained from embedded sensors.
Wt male C57BL/6J whole brains for multiple age time points were obtained, fixed and embedded in paraffin and were sectioned (10 µm) for embryonic day 15 (E15), post-natal day 1 (P14, P28, P28 and P70, liver, thymus, kidney and spleen were also taken from a P70 mouse.
